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基于纳米抗体的竞争 ELISA 法检测猪流行性腹泻病毒血清中和抗体的 ...
目前,虽然部分国家有疫苗可用,但 PED 依然严重威胁着全球猪产业。在诊断 PEDV 感染方面,现有的检测方法存在诸多问题。例如,传统的酶联免疫吸附试验(ELISA)会与其他猪冠状病毒抗体发生交叉反应,无法准确检测中和抗体(nAbs);而基于多克隆抗体和单克隆抗体的检测方法,不仅生产成本高、技术要求高,还耗时费力,并且抗体标记的批间差异影响检测稳定性和重复性。
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