数字PCR(Digital PCR-dPCR)技术是近年来出现的一种新的核酸检测和定量方法,与传统定量PCR(qPCR)技术不同,数字PCR采用绝对定量的方式,不依赖于标准曲线和参照样本,直接检测目标序列的拷贝数。由于这种检测方式具有比传统qPCR更加出色的灵敏度和特异性 ...
He needed a more sensitive detection method and turned to digital PCR (dPCR), discovering that it was able to detect viruses in samples that were negative with qPCR 2, 3. The principle behind dPCR ...
This infographic breaks down the technology behind each technique, as well as their workflows, applications and important the ...
A significant number of PCR systems, for example ... Image Credit: Roche Diagnostics The Digital LightCycler ® dPCR System is a new dPCR-based method for DNA methylation analysis.
expanding the utility of nanoplate digital PCR (dPCR) across various applications, including wastewater testing and human pathogen analysis. The first tool, the 'Design tool for Custom dPCR ...
real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.