The genetic connections between DNA repair pathways and human cancer predisposition have fueled interest in the proteins that recognize and repair specific sites of DNA damage. The repair enzymes are ...

7| Add to the reaction 1 μl of 50 mM β-nicotinamide adenine dinucleotide (β-NAD) and 1 μl E. coli DNA ligase (1,000–4,000 units/ml). Incubate the reaction at 15–25 °C for 15 min.