PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

Cell line development plays a pivotal role in the manufacturing of therapeutic proteins, vaccines and other biologics.

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

Overlay PCR reaction with 15 μl of mineral oil to ... rotate the to 96-well plate half way through the mixing process. Assess the quality of the emulsions at 400× magnification with an inverted ...

It also earned its inventor, Kary B. Mullis, the Nobel Prize in Chemistry in 1993. Before PCR, the process of DNA sequencing was very lengthy and quite expensive. How did the discovery of the Taq ...

One to twenty-four samples can be processed in a single run to isolate free-circulating DNA from fetal-originated DNA in ...