Formation of a phosphodiester bond releases the fluorophore, which quickly diffuses away, reducing fluorescence to background levels and generating a natural, unmodified DNA product (Fig.

Adjacent ribose nucleotide bases are chemically attached to one another in a chain via chemical bonds called phosphodiester bonds. Unlike DNA, RNA is usually single-stranded. Additionally ...

My scientific interests center on nucleases, a functionally diverse class of enzymes that catalyze the cleavage of phosphodiester bonds to support a wide breadth of pathways, including DNA replication ...