Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called ...

Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid DNA you may use the “divide by 20 rule” where you divide the size of the plasmid by 20 to determine the nanograms ...

“Charles River has decades of success reliably manufacturing plasmid DNA, viral vectors and cell ... "Charles River and ...

During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...

Prepare plasmid DNA using standard miniprep protocols ... through 0.8–1% agarose and determine the concentration of vector DNA by measuring UV absorbance at 260 nm or by running an aliquot ...

This cassette is then transferred to the adenovirus vector plasmid (pAdeno X, Clontech Laboratories or pAd5F35 ... Several plaques are selected, expanded, HIRT DNA is analyzed, and virus lysates are ...

Immunizing the host with a piece of viral DNA rather than an antigenic protein fragment of the virus, helps to stimulate the generation of cell-mediated immunity (Fig. 2). DNA vaccines contain the ...