THE RISE in ammonia levels in the Yamuna river due to pollution entering from Panipat and Sonipat was discussed by the Upper Yamuna River Board (UYRB) in December 2023, with the Member-Secretary of ...
After adjusting the pH to 8.5 with 1 M ammonium bicarbonate (AmBic), the ... After digestion with Endoproteinase Lys-C as described 19, the solution was diluted to 2 M urea with 100 mM AmBic ...
After evaporating to dryness, the hydrolysate was re-dissolved in water to yield a 1 mg/ml final solution. The ninhydrin reagent was prepared as follows: 400 mg ninhydrin ...
Simultaneously, sediment at a depth of 0–10 cm was collected by a mud bucket and stored at −80°C before analysis. 2 g dry sediment samples were extracted by 15 ml EDTA buffer solution and 15 ml ...
The enzyme was immobilized using the sodium alginate matrix technique. 21 One milliliter of cell-free extract (9000 U) was suspended in 9 mL of 2% (w/v) sodium alginate prepared in 50 mM Tris-HCl ...