MseI and EcoRI generate DNA fragments with 5′ overhangs (5′-TA-3′ and 5′-AATT-3′, respectively) that are distinct from each other and are noncomplementary. The aforementioned ligation ...

Figure 2: Ligation of circularizable probes by Ampligase, a thermostable DNA ligase. To facilitate allele discrimination in genomic DNA, we designed a second oligonucleotide primer to bind ...