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Cells were lysed in RIPA buffer (150 mM sodium chloride, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, and 50 mM Tris-HCl, pH 8.0), supplemented with MS-SAFE protease and ...

Researchers found that a solution of sodium chloride tablets and water helped athletes retain fluid during exercise, compared with athletes who used an alternative glycerol-based solution.