A surprising aspect of the results presented by Chechik et al. is the very late onset of NADPH regeneration: they indicate that most PPP enzymes are induced 4 to 7 minutes—or even later—after ...

Ltd.). SDS-PAGE gel electrophoresis preparation kit, BCA protein quantitative kit (Shanghai Beyotime Biotechnology Co., Ltd.). Mouse anti-vascular endothelial growth factor (VEGF), ...

Chronic exposure to hyperglycemia can lead to cellular dysfunction that may become irreversible over time, a process that is termed glucose toxicity. Our perspective about glucose toxicity as it ...

2 Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China 3 State Key Laboratory of Liver Research, Li Ka Shing Faculty of Medicine, University of ...

Target gene expression was determined relative to endogenous controls using the comparative cycle threshold method normalised to glyceraldehyde 3-phosphatase dehydrogenase (GAPDH) or β-actin ...

The autophagy lysosomal system (ALS) is crucial for cellular homeostasis, contributing to maintain whole body health and alterations are associated with diseases like cancer or cardiovascular diseases ...

7 Protein extracts were separated by SDS-PAGE, transferred to PVDF or nitrocellulose membranes and probed with the following antibodies: anti-IκBα; anti-phospho-NF-κB p65; anti-FLAG (Cell Signaling, ...

The energy stored in ATP and NADPH helps turn these 3-PGA molecules into another three-carbon molecule called glyceraldehyde-3-phosphate, or G3P. While some of these G3P are used to restart the ...