This repository's scripts aid in the initial design of overlap-extension reverse-transcription PCR (OE-RT-PCR) as described in the papers above. generate_primers.py: This script takes a FASTA file of ...

The Primer Design Tool is a powerful bioinformatics application designed to help researchers and scientists create highly optimized primers for PCR (Polymerase Chain Reaction) experiments. Built using ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Isothermal PCR techniques ... led to three overlapping matrices that were analyzed for both genotypes (serum, rectal swab and lymph node). Lung, tonsil and spleen samples were only tested in genotype ...

Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...

New Era's new MLB Overlap hats for 2025 -- in which they put the logos of teams over the names of said teams -- have become a source of a lot of humor for baseball fans everywhere. It's not just ...

Alternative splicing (AS) of pre-mRNA plays a crucial role in tissue-specific gene regulation, with disease implications due to splicing defects. Predicting and manipulating AS can therefore uncover ...

The primer parameters were set as follows: PCR product size 90–200 bp, primer size 18–22 bp, primer melting temperatures (Tm) set to 58.0–62.0°C, primer GC content 40–60%, and other parameters as ...

Photoshop CC tutorial showing how to make the individual, overlapping, photo strip effect from a single photo. The photo panels are fully editable and can be repositioned, resized and rotated.

Design Alcoholic hepatitis (AH) human and animal liver samples and hepatocytes were ... chromatin immunoprecipitation and immunofluorescence assays were carried out in cell models. Results The miRNA ...