You have full access to this article via your institution. To demonstrate the power of PCR–mediated recombination and provide an example of the general utility of the method, we constructed ...

Tanuka Biswas is a science communication professional, with over a decade of experience in molecular biology, cancer biology, and content strategy. She earned her PhD in biomedical sciences at the ...

I therefore devised a single-tube, nested PCR method that is easy to handle and yields consistent results. The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the ...