Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance.

2022年1月3日 · Affinity chromatography is a type of liquid chromatography for the separation, purification or specific analysis of sample components. It utilizes the reversible biological interaction or molecular recognition called affinity which refers to the attracting forced exerted in different degrees between atoms which cause them to remain in combination.

Affinity chromatography is a method of separating biochemical mixture based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and …

亲和层析(Affinity Chromatography)是利用生物分子间专一的亲和力而进行分离的一种层析技术。 人们很早就认识到 蛋白质 、酶等生物大分子物质能和某些相对应的分子专一而可逆的结合，可以用于对生物分子的分离纯化。

Affinity chromatography is one of the most diverse and powerful chromatographic methods for purification of a specific molecule or a group of molecules from complex mixtures. It is based on highly specific biological interactions between two molecules, such as interactions between enzyme and substra …

Affinity chromatography is a separation method based on a specific binding interaction between an immobilized ligand and its binding partner. Examples include antibody/antigen, enzyme/substrate, and enzyme/inhibitor interactions.

Affinity chromatography is a form of liquid chromatography that uses a biologically-related binding agent as the stationary phase [1 – 5]. This technique has been used for decades for the isolation and purification of specific targets by taking advantage of the selective and reversible binding which occurs in many biological interactions [4 – 7].

2024年3月18日 · Affinity chromatography separates proteins based on the reversible interaction between a protein (or set of proteins) and a specific ligand bound to the chromatographic matrix. Affinity chromatography makes purifications that would otherwise be time-consuming, difficult, or even impossible to accomplish with other techniques.

2024年8月7日 · Affinity chromatography excels in the domain of protein purification, offering unparalleled specificity and efficiency. The method’s ability to isolate proteins based on binding affinities ensures that the purified product is of high purity, often achieving in a single step what other techniques may require multiple rounds to accomplish.

Affinity chromatography is primarily used to capture a specific protein or a class of proteins within complex mixtures. The principle of affinity chromatography is based on the ability of a biologically active molecule to bind specifically and reversibly to a complementary molecule, which is often bound to a solid support.