Explore the light paths in Nikon's SMZ1500 stereomicroscope equipped for fluorescence illumination using an intermediate tube and external lamphouse. The Nikon blue excitation fluorescence filter combinations include bandpass and longpass sets having both broad and narrow excitation bandwidths.

The modern fluorescence microscope combines the power of high performance optical components with computerized control of the instrument and digital image acquisition to achieve a level of sophistication that far exceeds that of simple observation by the human eye.

This gallery examines the fluorescence microscopy of both cells and tissues with a wide spectrum of fluorescent probes. The fluorescence microscope provides an interesting window into the world of the cell and is one of the biologist's favorite tools …

Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins. The large product range includes longpass filter cubes for observation of single-stained samples and bandpass filter cubes suitable for detecting specific ...

The ECLIPSE Ni series is Nikon's flagship upright microscope, boasting great optical performance and high system expandability. Combined with high-performance objectives, it can accurately visualize microstructure of the sample with high resolution.

TIRF (Total Internal Reflection Fluorescence) microscopy facilitates extremely high-sensitivity and high-contrast visualization of single molecules near the coverglass, without disturbing cellular activity, thereby enabling the tracking of biomolecules, and the study of their dynamic activity and interactions at the molecular level.

Nikon provides products featuring various super-resolution microscopy techniques. In fluorescence imaging with STORM (Stochastic Optical Reconstruction Microscopy), fluorescent molecules are excited, and the signals are measured by distinguishing neighboring fluorescent molecules by time differences.

The Eclipse 90i is useful for imaging fluorescent or chromagenic (chemically stained) samples such as H&E, immunohistochemistry (i.e. DAB, HRP), Gram, trichrome, etc. This microscope is equipped with blue, green and red filters for imaging common fluorecence dyes in these ranges.

Fluorescence imaging uses high intensity illumination to excite fluorescent molecules in the sample. When a molecule absorbs photons, electrons are excited to a higher energy level. As electrons ‘relax’ back to the ground-state, vibrational energy is lost and, as a result, the emission spectrum is shifted to longer wavelengths.

2025年1月26日 · Nikon fluorescence microscopes typically use epifluorescence or total internal reflection fluorescence (TIRF) techniques to visualize fluorescence in samples. They offer high sensitivity and resolution, and can be used to visualize a wide range of fluorophores, including dyes, proteins, and nanoparticles. ...