2021年5月12日 · In this work, we propose a method for preparation of phosphoramidites, where the phosphitylating reagent is immobilized on a solid support, which allows rapid phosphitylation of nucleosides...

2018年9月13日 · Phosphoramidite chemistry is the gold standard method for DNA synthesis that has been used in the industry for almost 35 years. Since its discovery, its simplicity and high efficiency have allowed large volumes of oligonucleotide sequences to be synthesized up to 200 base pairs in length.

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing. Opposite of biosynthesis, chemical synthesis proceeds in the 3' → 5' …

In this work, we propose a method for preparation of phosphoramidites, where the phosphitylating reagent is immobilized on a solid support, which allows rapid phosphitylation of nucleosides eluting through the solid support, immediately before submission to oligonucleotide synthesis.

The chemical synthesis of DNA and RNA is universally carried out using nucleoside phosphoramidites or H-phosphonates as synthons. This review focuses on the phosphorus chemistry behind these synthons and how it has been developed to generate ...

Phosphoramidite oligo synthesis proceeds in the 3'- to 5'-direction (opposite to the 5'- to 3'-direction of DNA biosynthesis in DNA replication). One nucleotide is added per synthesis cycle. The phosphoramidite DNA synthesis cycle consists of a series of steps outlined in Figure 1.

Using this approach, we obtained 10 different classes of modified nucleoside phosphoramidites suitable for the synthesis of oligonucleotides, including several noncanonical nucleotides found in natural RNA or DNA (e.g., m 6 A, i 6 A, m 1 A, g 6 A, m 3 C, m 4 C, m 3 U, m 1 G, and m 2 G).